DMEM (High glucose) / DMEM medium (high sugar) Glutamine 50ul Non-essential amino acid 500ul Sodium pyruvate 500ul B-mercaptoethanol Double antibody 50ul serum 7.5ml LIF 5ul 2-mercaptoethanol 5.5uM 1000X L-glutamine 2mM 100X LIF 1000U/mL 10000X Non-essential amino acid 100uM 100X Double antibody 100X Product categories of Storage Furniture, we have years of experience in the manufacture of furniture, cube,Floating Shelf and Bookcase suppliers/factory, wholesale high-quality products of Study Desk , Gaming Desk and Working Desk. We have the perfect after-sales service and technical support. We hope we can cooperate happily. Storage Furniture,Bathroom Storage,Shoe Storage Cabinet,Shoe Storage Bench Baklam , https://www.baklamfurniture.com
First, prepare DMEM serum medium
1. Take the FBS from the refrigerator at -20 °C one day in advance, and put it in a refrigerator at 4 °C;
2. FBS and other components to be added (such as sodium pyruvate, antibiotics, etc.) are added to the DMEM medium in proportion to the cells, and placed in a refrigerator at 4 °C.
The preparation ratio is as follows:
Second, ES cell culture medium
DMEM+15% FBS
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