Laboratory animal immunization protocol 1. Antigens: proteins, peptides, organelles, cells, tissues, etc. 2. Immunization method: subcutaneous injection, intraperitoneal injection, intravenous injection, intramuscular injection, etc. 3. The amount of antigens required for different animal immunizations (taking protein immunization as an example) > 18-20kDa <18-20kDa Animal antigen amount optimal antigen amount antigen amount optimal antigen amount Rabbit 20-200 100 50-400 200 Mouse 2-40 15 4-60 40 Rat 10-50 30 20-150 50 Sheep 100-1000 200 200-1000 400 Hen 20-200 100 50-400 200 Unit: microgram 4. Immunization plan (take immunization rabbit as an example): Days 0 14 28 38 56 66 87 Injection x x x x Blood collection 2ml 2ml 2 + 20ml 2 + 50-70ml 5. Note: 1) The complete adjuvant is mixed with the immunogen for the first immunization, and the incomplete adjuvant is mixed with the immunogen for the booster immunization. A multi-point, time-interval immunization method is adopted. 2) If the rabbit is immunized with cells, the amount of cells required for each immunization is 2-3 x 107 cells. 3) After three consecutive immunizations, a small amount of blood is required for ELISA detection to detect the antibody titer of the immunized animal, generally the titer can reach 1: 10,000-50,000. Immunization should be boosted once a week before the animals immunized. 4) If you are immunized with mice, you can collect a small amount of blood from the tail vein (about 50µl), and finally take a large amount of blood from the eyeball (about 500-1000µl); if you are immunized with a rabbit, you can take a small amount of blood from the ear vein (about 2mL), and finally Large blood collection of the heart (50-100mL) 5) Separate the serum according to the standard method of serum preparation, divide it into small portions, and store at -80oC. COMPLETE SET- This high quality set comes with everything needed for a successful cook out. Easily season steaks with the basting brush, flip hot dogs or burgers the spatula or tongs, eat corn with the corn holders or grill kabobs with the skewers.
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Laboratory animal immunization protocol