Due to the high catalytic efficiency of the enzyme, the results of the immune response are indirectly amplified, making the assay method highly sensitive. The required sample size depends on your test items. Some test items require a 1: 100 dilution of the serum, or even a higher dilution, then a few microliters of blood sample is enough, and some items can only do a lower dilution, even Do not dilute, in this case, each well will need about 100 ul. First of all, please pay attention to the expiration date when buying ELISA kits. Try to buy the ones that have just left the factory. Everyone knows more about the kit is nothing more than worrying about the success of the experiment, how to interpret the results after the kit comes out. As long as it is a commercial kit, it is relatively stable, but ELISA kits generally do not detect antigens as accurately as antibodies. Plastic Printing Ink,Pantone Color Ink,Screen Printing Ink,Metal Printing Ink shaoxing gesheng wire mesh co., ltd , https://www.gscreenmesh.com
During the measurement, the test specimen (the antibody or antigen in it) is reacted with the antigen or antibody on the surface of the solid phase carrier. After adding the substrate for the enzyme reaction, the substrate is catalyzed by the enzyme into a colored product. The amount of the product is directly related to the amount of the test substance in the specimen, so qualitative or quantitative analysis can be performed according to the depth of the color. The basis of ELISA is the solid phase of antigen or antibody and the enzyme labeling of antigen or antibody. Before buying, please communicate with the technical personnel in detail. Some products are classified very fine. There will be several kits for the same project. The antigen-antibody complex formed on the solid phase carrier is separated from other substances in the liquid by washing, and then the enzyme-labeled antigen or antibody is added, and is also bound to the solid phase carrier through the reaction. At this time, the amount of enzyme on the solid phase is proportional to the amount of test substance in the specimen. The antigen or antibody bound to the surface of the solid phase carrier still maintains its immunological activity, and the enzyme-labeled antigen or antibody preserves both its immunological activity and enzyme activity. Regarding the relationship between anticoagulation and the volume of serum plasma obtained, roughly the ratio is 1 ml of whole blood to obtain 500 ul of serum or plasma.