How to use the medical centrifuge of Kyushu Space Analysis Laboratory

How to use laboratory medical centrifuges? Medical centrifuges are widely used in medical research or hospital testing. At present, centrifuge products with more applications in hospitals include blood centrifuges, blood card centrifuges, urine sediment centrifuges, and biopharmaceuticals. Centrifuges, gel bubble processing centrifuges, etc., have a wide variety of functions and functions. This article briefly introduces the use, function, application type, use and management and maintenance specifications of medical centrifuges.

(1) Medical centrifuge use and function:

1. For separating particles suspended in a solution

1) Separating the formed components in the blood, and concentrating the cells or other formed components in the body fluid for analysis and determination.

2) Isolation of a ligand that binds to a protein or an antibody, a free ligand, and the like.

3) Separation of proteins that have been precipitated in the specimen.

2. Separation of two different density incompatible liquids

1) If an organic solvent is used to extract certain components of the body fluid.

2) Separation of lipid components in the blood, such as separation of chylomicrons and various lipoproteins in plasma.

(2) Types of medical centrifuge applications

1. Horizontal rotary head or bucket type.

2. Fixed angle rotor or angle rotor

3. High speed centrifugal type.

The above three categories all contain floor-standing, bench-top

(3) Principle of centrifugation

Relative centrifugal force (RCF) is used to express its centrifugal force.

RCF=1.118×10-5×r×K2

Where: 1.118×10-5 is the experimental constant, r is the radius from the center of the shaft to the bottom of the centrifuge tube (cm); n is the rotation speed.

[4] Medical centrifuge operation

In order to ensure the normal operation of the centrifuge, the centrifuge tube supplied by the manufacturer must be used, and the two ends of the rotor shaft are symmetrically separated.

The heart tube, tube rack, and centrifugal bucket must be balanced. The maximum load capacity at both ends during centrifugation shall not exceed the limit specified by the manufacturer.

degree. The use of high-speed centrifuges must be carried out by a dedicated person, and the technician reviews the speed and the rotor used.

(5) Medical centrifuge management and maintenance

1. If the centrifuge tube or empty tube of the specimen is broken during centrifugation, the broken glass should be removed immediately and centrifuged with disinfectant.

Disinfect the bucket or the rotating hole of the rotor. In case of radioactive contamination, the pollution shall be removed and recorded as required.

2. Use a damp cloth to rub the inside and outside of the centrifuge at least once a week.

3. Check the balance of the centrifuge every 3 months and add lubricant once.

4. Check the carbon brush every 3 months and find that it is used up and replaced in time.

5. Check the speed every 3 months to match the indicator.