With the rapid development of 3D printing technology , 3D printing has penetrated into the medical field. Today, a breakthrough diagnostic tool, the use of enzyme-linked immunosorbent assay (ELISA), makes medical institutions in modern society quite effective. At present, when it comes to the diagnosis of related viruses and bacteria, such as hepatitis, West Nile virus, HIV, herpes, measles and mumps, the application of ELISA can usually be completed within a few days, only Blood is taken and specific antigens are tested using large equipment. However, current ELISA machines are very large and are usually found in some large clinical laboratories. And those developing countries or poor areas are often unable to buy and install such machines, but the need to detect viruses and bacteria in these areas is even more urgent. It can be said that if there are fast and accurate detection instruments like ELISA in developing countries, it may save millions of lives. In order to solve this problem, researchers at the California Institute of Nanosystems at the University of California, Los Angeles (UCLA) have developed a new 3D printing smartphone device that can quickly read only those large and expensive machines. Take the ELISA tray. In a report entitled "Cellphone-Based Hand-Held Microplate Reader for Point-of-Care Testing of Enzyme-Linked Immunosorbent Assays" Under the leadership of Aydogan Ozcan, deputy director of the California Institute of Nanosystems, Dino Di Carlo, professor of bioengineering, and Omai Garner, deputy director of clinical microbiology at UCLA Medical Systems, the researchers introduced this new, highly accurate and accurate method. Device. “ELISA-based standard microplates are widely used in a variety of nanomedicine, molecular sensing, and disease screening applications, and batch analysis of such microplates is significantly reduced compared to non-batch/non-standard tests. The cost of diagnosis for each patient." Research points out, "However, their use is limited by the need for some large and expensive reading instruments. To alleviate this problem, we have created a low-cost handheld ratio based on mobile phones. A microplate reader that uses a 3D printed optomechanical machine to secure a 96-well plate and illuminate it with an array of LEDs." The device currently made by researchers is based on a Nokia Lumia 1020 smartphone. It is understood that this phone is equipped with a 3D printed attachment and connected to the UCLA server via the cloud. It illuminates the ELISA plate with an LED array. Light then passes through the 96 apertures on the aperture plate and enters the 96 fibers below the aperture, and the collected data is sent to the UCLA server via a customized app on the phone. The server then uses a special learning algorithm to detect the image received by each well, then calculates the mean, standard deviation, maximum, minimum, and maximum and minimum differences and submits the results to the medical professional. "We successfully performed ELISA testing of this mobile platform using a FDA-approved mumps virus IgG, measles virus IgG antibody, and herpes simplex virus IgG (HSV-1 and HSV-2) in a clinical health research institute. The test used 567 and 571 patient samples for general testing and blind testing, respectively, for the above four viruses (mumps virus, measles virus, HSV-1 and HSV-2), respectively, 99.6%, 98.6% , 99.4% and 99.4% accuracy," the researchers wrote. "This cost-effective handheld platform can help healthcare professionals prevent high-risk disease or track vaccination through on-site testing, even in resource-poor areas." Devices that look fairly simple, but effectively combine 3D printing, smartphones and cloud computing technology, have achieved quite amazing results. It enables the latest medical technology to be enjoyed in remote areas. The research team hopes to extend this reliable diagnostic tool to all developing regions of the world for the benefit of local people.
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