Enzyme-linked immunoassay of human total hemolytic complement (CH50) Kit instruction manual This reagent is for research purpose only: This kit is used to determine the total hemolytic complement (CH50) in human serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of total human hemolytic complement (CH50) in the specimen. The microplate was coated with purified human total hemolytic complement (CH50) antibody to make a solid-phase antibody. The total hemolytic complement (CH50) was added to the monoclonal antibody-coated microwells in turn, followed by HRP labeled total hemolytic complement (CH50) ) The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the total hemolytic complement (CH50) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of total human hemolytic complement (CH50) in the sample was calculated by a standard curve. Clear and not greasy sunscreen,Rapid film formation sunscreen,Easy to clean sunscreen,Waterproof and sweat resistant isolation milk HomeSafe Group , https://www.homesafebio.com