A Comparative Study on Peaking of Gentamicin Sulfate

Posted on August 18, 2024

A Comparative Study on Peaking of Gentamicin Sulfate
1. Experimental method
Cometro high performance liquid chromatography system;
Chromatography column: Inertex C18 150 * 4.6mm, 5Î¼m;
Evaporative light scattering detector (SOFTA M300S): SC 30 â„ƒ DT 70 â„ƒ CL 20 â„ƒ; Gain: Normal;
Flow rate: 1.0ml / min, 0.8ml / min, 0.6ml / min;
Sample: Gentamicin sulfate sample 2.5mg / ml;
2. Experimental results
1. Flow rate 1.0ml / min
2. Flow rate 0.8ml / min
3. Flow rate 0.6ml / min
3. Conclusion
It can be seen from the comparison of different flow rates that the best effect is achieved when the flow rate is 1 ml / min when all samples can be completely separated.

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