The pH of the rhizosphere affects the absorption and accumulation of plant nutrients, as well as the activity of microorganisms, the rate of mineralization, and the exchange of ions. The extracellular pH affects the potential difference between the plasma membrane and the surrounding environment, which in turn affects the amount and activity of H + -ATPase on the cell membrane, which will affect the transport of inorganic nitrogen. The mechanism of NH4 + and NO3-transport is different under different pH conditions. The current model believes that nitrogen absorption is through the low affinity transport system (LATS) and the high affinity transport system (HATS), which are closely related to protons. In 2010, Canadian scientists used non-damaging micro-measurement technology to study the absorption process of NH4 + and NO3- in the coniferous tree and soybean roots under the influence of pH. Many species of conifers grow in acid soils, but their preferences for 4+ and NO3- are very different, and pH affects the absorption of nitrogen. The NH4 +, NO3- and proton flow rates of different parts of the root (root tip, 5mm, 10mm, 20mm and 30mm from the root tip) were measured. The measurement conditions were 50μM and 1500μM NH4NO3, and the pH was 4 and 7. In soybean and twisted pine, protons flow out at pH 7, but protons flow in at pH 4. Proton outflow occurs behind the root tips of Douglas Fir. Douglas Fir can maintain NH4 + absorption at low pH, which may be related to maintaining proton outflow. This proton outflow makes plants better adapt to acid soils. Among these three materials, the absorption of NO3- is the largest at neutral pH, especially in high nitrogen. Therefore, nitrogen concentration is the main factor that determines the absorption of nitrogen at a suitable pH. In Douglas fir and soybean, nitrogen absorption is greater in 1500 μM than in 50 μM NHH4NO3, but the proton flow pattern is different in each species. The results of this ion current provide a pattern of nitrogen uptake in space and time, indicating that plants have different nutrient absorption mechanisms. Keywords: High-affinity transport system (HATS); low-affinity transport system (LATS); non-damaging micro-measurement technology (MIFE); nitrogen (N, nitrogen) references : Hawkins BJ and Robbins S. Physiologia Plantarum, 2010, 138: 238-247.

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